The expression of ecdysteroid UDP-glucosyltransferase enhances cocoon shell ratio by reducing ecdysteroid titre in last-instar larvae of silkworm, Bombyx mori
نویسندگان
چکیده
منابع مشابه
Expression and structural characterization of a baculovirus ecdysteroid UDP-glucosyltransferase.
The baculovirus enzyme ecdysteroid UDP-glucosyltransferase (EGT) disrupts the hormonal balance of the insect host by catalysing the conjugation of ecdysteroids, the moulting hormones, with the sugar moiety from UDP-glucose or UDP-galactose. In this study, EGT has been overproduced using a recombinant Autographa californica nucleopolyhedrovirus and an antiserum has been raised against the purifi...
متن کاملThe ecdysteroid UDP-glucosyltransferase gene of Autographa californica nucleopolyhedrovirus alters the moulting and metamorphosis of a non-target insect, the silkworm, Bombyx mori (Lepidoptera, Bombycidae).
The Autographa californica nucleopolyhedrovirus (AcMNPV) does not infect the silkworm and molecular studies on silkworm insusceptibility have not been performed. In cultured cells of the silkworm, the expression of viral genes has been reported. The expression of AcMNPV genes and their effect in vivo and in vitro was studied. In this study, the early gene, the ecdysteroid UDP-glucosyltransferas...
متن کاملPurification and kinetic analysis of a baculovirus ecdysteroid UDP-glucosyltransferase.
The baculovirus ecdysteroid UDP-glucosyltransferase (EGT) disrupts the hormonal balance of the insect host by catalysing the conjugation of ecdysteroids, the moulting hormones, with the sugar moiety from UDP-glucose or UDP-galactose. In this study, Autographa californica nucleopolyhedrovirus EGT has been overproduced and purified, and its kinetic properties determined. The enzyme was purified 1...
متن کاملتأثیر غنی‌سازی برگ توت با ویتامین ریبوفلاوین بر عملکرد اقتصادی و باروری کرم ابریشم .Bombyx mori Lدر استان گیلان
The effect of mulberry leaves enrichment with riboflavin in 7, 37, 77 and 127ppm concentrations on larval growth and cocoon characteristics of Bombyx mori L., hybrid 103×104 was studied. Silkworm larvae were fed on fresh mulberry leaves of shin inche nevise enriched with riboflavin once a day. All biological and economical parameters were determined by using standard techniques in sericulture. ...
متن کاملتأثیر غنی‌سازی برگ توت با ویتامین ریبوفلاوین بر عملکرد اقتصادی و باروری کرم ابریشم .Bombyx mori Lدر استان گیلان
The effect of mulberry leaves enrichment with riboflavin in 7, 37, 77 and 127ppm concentrations on larval growth and cocoon characteristics of Bombyx mori L., hybrid 103×104 was studied. Silkworm larvae were fed on fresh mulberry leaves of shin inche nevise enriched with riboflavin once a day. All biological and economical parameters were determined by using standard techniques in sericulture. ...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-36261-y